Core technology

After mitochondrial transformation of a Rho° yeast strain using a biolistic gun, mitochondria of the transformant strain are able to produce a unique RNA through DNA transcription.

Yeast mitochondria are also exploited as RNA production and storage micro-units.

The culture of the transformed strain on a sugar containing growth medium allows exponential multiplication of these RNA production and stocking micro-units.

The strain also utilizes sugar to synthesize its production and storage machinery, and to produce RNA.

The mitochondrial compartment can then be easily recovered through cellular fractionation.

At last, the unique RNA produced in mitochondria can be simply purified, given the fact that it is the only RNA present in the mitochondrial fraction.